HLA B-27 by PCR Test: Booking, Price, and Results
About HLA B-27 by PCR Test
| Field | Value |
|---|---|
| Also Known As | HLA-B27 PCR, Human Leukocyte Antigen B27 Test, B27 Antigen Test, HLA B27 Qualitative PCR |
| Sample Type | Venous whole blood (EDTA tube) |
| Fasting Required | No fasting required |
| Report Time | 4 to 8 days |
| Recommended For | All genders and age groups, particularly males in late adolescence or early adulthood, with suspected autoimmune conditions |
| Price | Starting at ₹2,700 |
What is an HLA B-27 by PCR Test?
The HLA B-27 by PCR test checks for the presence of a specific protein called Human Leukocyte Antigen B27 on the surface of white blood cells. Doctors order this test when a patient has symptoms suggesting certain inflammatory or autoimmune conditions. A small blood sample is collected from a vein in the arm. This test is also known as the human leukocyte antigen B27 test, HLA-B27 PCR, or B27 Antigen Test.
What Does a HLA B-27 by PCR Test Measure?
This test uses polymerase chain reaction (PCR), a laboratory method that amplifies and analyses DNA, to detect the presence of the HLA-B27 gene. PCR is considered more sensitive than older methods such as flow cytometry.
The test identifies one key marker:
| Component | What It Represents |
|---|---|
| HLA-B27 Protein/Gene | A genetic marker found on white blood cells that is associated with a higher likelihood of certain autoimmune and inflammatory conditions |
The result is reported as either detected (positive) or not detected (negative).
Why is an HLA B-27 by PCR Test Done?
A doctor may order the HLA B-27 by PCR test when a patient presents with specific signs that suggest an autoimmune or inflammatory condition.
Common Symptoms That May Require This Test
Several symptoms may prompt your doctor to request this test. These include:
- Chronic or persistent back pain, especially in younger adults
- Joint pain and stiffness, particularly in the morning
- Swelling or inflammation in the joints
- Recurring eye inflammation (uveitis), causing redness or blurred vision
- Inflammation of the urethra alongside joint symptoms
- A family history of autoimmune or inflammatory conditions
Conditions This Test Can Help Detect
The Human Leukocyte Antigen B27 test is used to support the diagnosis of several conditions. These include:
- Ankylosing spondylitis, a chronic inflammation affecting the spine and sacroiliac joints
- Reactive arthritis, which occurs after infections in the gut or urinary tract
- Psoriatic arthritis, a condition seen in people who also have the skin condition psoriasis
- Enteropathic arthritis, linked to inflammatory bowel diseases such as Crohn's disease and ulcerative colitis
- Acute anterior uveitis, a recurring inflammatory condition of the eye
- Juvenile idiopathic arthritis, a form of arthritis affecting children
- Assessment of tissue compatibility for organ transplantation
How to Prepare and What to Expect
Preparation for this test is straightforward. No special steps are needed before your appointment.
Do You Need to Fast?
No. Fasting is not required before the HLA B-27 by PCR test. You may eat and drink as you normally would before your blood draw. If your doctor has ordered other tests at the same time, check whether any of those require fasting.
Practical Tips Before Your Test
A few simple steps can help make your experience smooth. Keep the following in mind:
- Tell your doctor about any medicines you are currently taking, as some may affect the quality of the sample.
- Wear a short-sleeved top or loose-fitting clothing to make the blood draw easier.
- Try to stay calm and relaxed before the procedure.
- If you have an active infection or feel unwell on the day, consider rescheduling after speaking with your doctor.
Step-by-Step Procedure
Here is what typically happens during sample collection:
- A phlebotomist (a trained blood-collection professional) will clean the area on your arm with an antiseptic.
- A small needle is used to draw blood from a vein, usually at the inner elbow.
- The blood is collected in a lavender-top EDTA tube, which contains an anticoagulant that prevents clotting.
- The sample is labelled and kept at room temperature for transport to the laboratory.
- In the lab, PCR is used to detect the presence of the HLA-B27 gene.
- Results are prepared and reported as positive (detected) or negative (not detected).
Factors That Can Affect Accuracy
Certain conditions can affect the quality of your sample. These include:
- Haemolysis (breakdown of red blood cells in the sample)
- Use of an incorrect blood collection tube
- A clotted or frozen specimen
- Samples older than 72 hours from the time of collection
- Active infections at the time of testing, which may affect sample quality
Understanding Your HLA B-27 by PCR Test Results
Your results should always be reviewed alongside your symptoms and full medical history. A doctor is best placed to interpret what the outcome means for you personally.
| Parameter | Result | Interpretation |
|---|---|---|
| HLA-B27 | Negative (Not Detected) | The HLA-B27 gene is absent, which lowers the likelihood of associated conditions, though it does not fully rule them out |
| HLA-B27 | Positive (Detected) | The HLA-B27 gene is present, associated with a higher risk of certain autoimmune conditions |
Note: Approximately 10% of the general population tests positive for HLA-B27 without developing any related disease. The marker is found in 80 to 95% of people with ankylosing spondylitis, but not everyone who tests positive will develop this or any other condition.
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
How to Maintain Healthy Levels
Since HLA-B27 is a fixed genetic marker, the result itself does not change. However, if you carry the marker or have a related condition, these general habits may support your well-being:
- Staying physically active with regular, low-impact exercise can help maintain joint flexibility and reduce stiffness
- A balanced diet rich in vegetables, whole grains, and lean protein supports overall immune health
- Managing stress through relaxation techniques such as yoga or breathing exercises may help ease symptoms associated with inflammatory conditions
Lupin Diagnostics HLA B-27 by PCR Test Price and Home Collection
The HLA B-27 by PCR test is available at Lupin Diagnostics with home sample collection across major Indian cities. Prices vary by location, as shown in the table below:
| City | Approximate Price (₹) |
|---|---|
| Mumbai | ₹2,700 |
| Hyderabad | ₹2,700 |
| Chennai | ₹2,700 |
| Bangalore | ₹2,700 |
| Kolkata | ₹2,700 |
| Pune | ₹2,700 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Home Collection
Lupin Diagnostics offers home sample collection for the human leukocyte antigen B27 test across cities in India. All samples are processed in NABL-accredited laboratories by experienced technologists. Your digital report is shared via email or WhatsApp once it is ready, so you can access it from anywhere.
Frequently Asked Questions
The HLA B-27 by PCR test is ordered to help a doctor confirm or investigate a suspected diagnosis of conditions such as ankylosing spondylitis, reactive arthritis, juvenile idiopathic arthritis, or recurring eye inflammation. It is a supporting tool, used alongside clinical examination and other tests.
No. A positive result means you carry the HLA-B27 genetic marker, which is linked to a higher risk of certain conditions. Many people who test positive remain entirely healthy throughout their lives. Your doctor will assess this result in the context of your symptoms and overall health.
A negative result lowers the likelihood of HLA-B27-associated conditions, but it does not fully exclude them. Some people without the gene can still develop these conditions. Your doctor will consider your clinical history alongside the result.
No fasting is needed. You can eat and drink normally before your appointment. If your doctor has ordered additional tests at the same time, check with them whether any of those require fasting.
Results for the HLA B-27 by PCR test typically take 4 to 8 days. PCR-based testing requires specialised equipment and expertise, which is why its turnaround time is longer than that of routine blood tests.
Repeat testing is rarely necessary. Your HLA-B27 status is determined by your genes and does not change over time. If your doctor needs to monitor disease activity, they will use other markers such as ESR or CRP rather than repeat the HLA-B27 test.
Yes. The procedure involves a standard blood draw and is considered safe for most people. You may experience mild discomfort, slight bruising, or minor bleeding at the collection site. These effects are temporary and resolve quickly on their own.
HLA B-27 by PCR Test
